HIV INVIVO HUMAN CLINICAL STUDIES

In the Invivo human clinical studies, this drug were provided as a treatment to the HIV patients through HAKH Medical Foundation and the improvements in the patients in terms of physical as well as pathological were recorded in proper, controlled condition through standard procedure and all the blood test pertaining to HIV were undertaken through Molecular Diagnostic Services in association with Reliance Life Science and the reports & blood samples were maintained for more than 3-5 yrs for repeatability.

The clinical observation of this drug revealed the following improvements in the patients:

• CD4 count doubled in the patient and CD8 count declined to 30-50%.
• 7-8 folds decrease in HIV viral load counts within 5-6 weeks of the treatment.
• Viral load count decreased to less than detectable limit (>100) within 5-7 months of the treatment.
• 4-6 pounds increment in weight within 2-4 months.
• Relief from HIV symptoms.
• Prophylactic against opportunistic infections.

1. Phenomenal change in DNA-PCR Antigen test Patients those continued the treatment for 18-20 months after attaining the viral load to less than detectable limit and adhering to instruction strictly had recorded HIV DNA-PCR Antigen Qualitative test ’Not Detected’ and the test interpreted ‘the blood sample to be negative for the presence of HIV”. And so the medication were completely stopped for almost one year and again undergone for HIV DNA-PCR Antigen test (Qualitative) and recorded ‘Not Detected’. The same test was repeated for 2nd, 3rd & 4th year after completely stopping the medications and the patients recorded ‘Not Detected’ for the test. The method for the confirming the presence of HIV antigen is HIV DNA-PCR Antigen test (Qualitative) test by Nested PCR Technique.
2. A major breakthrough on HIV research through Antigen Vs Antibody Test. HIV antibodies remain in the body for a long time to come although HIV antigen is completely evaded from the body.

Methods : 6ml serum sample is to be drawn from the HIV patients who were earlier confirmed HIV positive through Western blot test and later undergone complete treatment with Unani medicine HOO-IMM PLUS. The sample is to be cautiously handled as per the NABL guidelines and to be sent to a renowned and reliable PCR Molecular diagnostic lab. 3 ml of the sample is to be allocated for Western Blot Antibody test and the other 3 ml for HIV DNA-PCR Antigen test.

Results : Our study showed that the above mentioned tests showed that the blood sample is positive for Western blot Antibody test & Negative for HIV DNA-PCR Antigen test. This directly interprets that at present the person is HIV negative though he still carries HIV antibodies authenticating his earlier infection with HIV. Conclusion: It could be concluded that our body immune system creates cell mediated immunity responses towards HIV antigen to produce antibodies that may persist for a long time, even after the antigen is completely eliminated from the body. The other best example is Mycobacterium Tuberculosis antibodies. The human body can respond to antigen in many different ways.

These fall into two major categories:

• Antibody-mediated immunity. Antibodies, dissolved in blood, lymph, and other body fluids bind the antigen and trigger a response to it. (This form of immunity is also called humoral immunity).
• Cell-mediated immunity (CMI). T cells (lymphocytes) bind to the surface of other cells, display the antigen and trigger a response and may remain in the body for a long time. The response may involve a) other lymphocytes and b) a ny of the other white blood cells (leukocytes)