Composition, Dosage & Direction

The HOO-IMM PLUS ™ (A, B, C, D, E) is an absolutely new, novel,pioneering and revolutionary concept from HOOTONE REMEDIESto medical science. It is a very safe, efficacious Indian medicine for the relief of AIDS and Hepatitis according to the degree and stageof affliction. This our herbal preparation has not only recorded the highest inhibition rate i.e. 98% against the causative agent HIV virus in invitro Anti-retroviral activity- drug sensitivity .

HOO-IMM PLUS works on our pioneering principle :-
• Inhibits viral multiplication.
• Augments production of CD4 lymphocytes cell.
• Immuno Restorative
• Treatment & Prophylaxis for Opportunistic Infections.
• Stimulate & sustain physiological activity in the body.

How HOO-IMM PLUS acts for HIV/ AIDS:

• Decreases RNA viral load up to undetectable level.
• Increases CD4 Helper T cells.
• Tones up functions of vital organs of the body.
• Curtail the development of ailments.
• Reduce & stop the vulnerability to opportunistic infections.
• Not prone to Multi drug resistance.
• It is safe & free from side effects & contra-indications.
• Completely free from any of the injurious ingredients like lead or steroid.
•  Immunity, vigour & vitality of the patient is restored enabling him to lead a productive energetic life.
•  It is very efficacious as a prophylactic to prevent transmission of HIV virus from mother to foetus during pregnancy.

LAB OBSERVATION	CLINICAL OBSERVATION	MOLECULAR DIAGNOSTICS
INVITRO TESTING	INVIVO TESTING	ANTIGEN TESTING
98% inhibition against the causative agent HIV virus has been recorded in the Anti-RetroviralActivity Testing Drug Sensitivity Testing made in a lab Studies, revealing the highest inhibition rate among any anti-retroviral medicines against HIV.	70% decrease in RNA viral load Realtime RT-PCR within first month of the treatment, and less than detectable level within 3 months of the treatment in the patients who were earlier possessing very high viral load counts of more than 1 lakh - 2 lakh copies/ml	The patients who were earlier confirmed positive through Western blot antibodies test later after the treatment with this medicines has recorded ‘Not Detected’ in DNA-PCR Antigen test ie. The Sample of the patient is Negative for the presence of HIV virus

Safety data from animal studies, Steroids & Metal free Certified.

The Toxological Studies, Storage Stability Studies and are being successfully made from the FDA Approved Lab. These medicines proved to be free from any inclusion of Steroids or Heavy Metals.

Also the Certificate of test or analysis by Government analyst and from Drug Control Laboratory Maharashtra State, Mumbai, reveals the safety of the drugs against all metal, steroids, chemicals and other harmful ingredients.

For better understanding of the drugs and its effective action against HIV virus one should undergo the below mentioned testing and undertake the studies from any renowned & well-equipped institution.

1) ARV Testing or Drug Sensitivity Testing to realize the inhibition rate of the drug against HIV.

2) HIV Phenotyping test to realize that the drug is completely free from multi-drug resistance.

3) Invivo Clinical studies to realize the improvement in CD4 cells & overall health of the patients.

4) RNA viral load / Realtime RT-PCR Quantitative testing to evaluate the rate of decrease in viral load in the patients in the 1st month, 2nd month and 3rd month of the treatment.

INVITRO STUDIES:

In invitro lab studies, this drug was put for Drug Sensitivity Test and Anti-retroviral Activity Test in the laboratory adhering to the standard protocol and the highest inhibition rate of 98% was recorded against the causative agent, HIV virus. The detail procedures undertaken in an invitro lab study are mentioned as under: -

Procedure of the invitro lab study

• Collect 20 ml Blood in an EDTA containing vial

• Mix it properly and keep at room temperature

• Overlay this blood sample on 2 ml Histopaque solution in
  25-ml sterile centrifuge tube. Centrifuge the tube at 1500 rpm for 20 min

• Two layers will be formed after centrifugation

• Separate out the upper layer of Histopaque   with the help
  of sterile pipette and collect the lymphocyte ring in 2 ml sterile eppendorf tube

• Microfuge it for 1 min at the bottom of the eppendorf tube lymphocytes sediment will be observed.

• Resuspend the sediment in 2-ml fresh RPMI-C Media.

• Prepare the serial dilutions of the drug of our interest or to be tested in the range of 1ug/ml – 500 ug/ml

• Add 500 ul of the lymphocyte culture with the help of sterile pipette / tips and add the appropriate dilution of the drug to the respective culture well along with control without addition of any drug.

• Incubate it in CO2 incubator at 37 degree Celsius (5% CO2)

• After incubation do the P-24 ELISA test for Control and for Test

• Interpret the results on the basis of difference in between the Control O.D and Test O.D and calculate the % Inhibition of the viral growth in Test in comparison with Control.

• O.D of the Control always ranges from 0.2-0.4 while the O.D of Test ranges in between 0.06-0.1, which indicates the inhibition of the virus multiplication.

INVIVO STUDIES:

In the Invivo human clinical studies, this drug were provided as a treatment to the HIV patients through HAKH Medical Foundation and the improvements in the patients in terms of physical as well as pathological were recorded in proper, controlled condition through standard procedure and all the blood test pertaining to HIV were undertaken through Molecular Diagnostic Services in association with Reliance Life Science and the reports & blood samples were
maintained for more than 3-5 yrs for repeatability.

The clinical observation of this drug revealed the following improvements
in the patients:

• 7-8 folds decrease in HIV viral load counts within 5-6 weeks
  of the treatment.

• Viral load count decreased to less than detectable limit (>100)
  within 5-7 months of the treatment.

• Augment in CD4 count noted.

• 4-6 pounds increment in weight within 2-4 months.

• Relief from HIV symptoms.

• Prophylactic against opportunistic infections.

Phenomenal change in DNA-PCR Antigen test

Patients those continued the treatment for 18-20 months after attaining the viral load to less than detectable limit and adhering to instruction strictly had recorded HIV DNA-PCR Antigen Qualitative test ’Not Detected’ and the test interpreted ‘the blood sample to be negative for the presence of HIV virus”. And so the medication were completely stopped for almost one year and again undergone for HIV DNA-PCR Antigen test (Qualitative) and recorded ‘Not Detected’. The same test was repeated for 2nd, 3rd & 4th year after completely
stopping the medications and the patients recorded ‘Not Detected’ for the test.

The method for the confirming the presence of HIV antigen is HIV DNA-PCR Antigen test (Qualitative) test by Nested PCR Technique.

A major breakthrough on HIV research through Antigen Vs
Antibody Test.

HIV antibodies remain in the body for a long time to come although HIV antigen is completely evaded from the body. This Methods: 6ml serum sample is to be drawn from the HIV patientswho were earlier confirmed HIV positive through Western blot test and later undergone complete treatment with Unani medicine HOO-IMM PLUS. The sample is to be cautiously handled as per the NABL guidelines and to be sent to a renowned and reliable PCR Molecular diagnostic lab. 3 ml of the sample is to be allocated for Western Blot Antibody test and the other 3 ml for HIV DNA-PCR Antigen test.

Results: Our study showed that the above mentioned tests showed that the blood sample is positive for Western blot Antibody test & Negative for HIV DNA-PCR Antigen test. This directly interprets that at present the person is HIV negative though he still carries HIV antibodies authenticating his earlier infection with HIV virus.

Conclusion: It could be concluded that our body immune system creates cell mediated immunity responses towards HIV antigen to produce antibodies that may persist for a long time, even after the antigen is completely eliminated from the body. The other best example is Mycobacterium Tuberculosis antibodies.

The human body can respond to antigen in many different ways. These fall into two major categories:

• Antibody-mediated immunity. Antibodies, dissolved in blood, lymph, and other body fluids bind the antigen and trigger a response to it. (This form of immunity is also called humoral immunity).

• Cell-mediated immunity (CMI). T cells (lymphocytes) bind to the surface of other cells, display the antigen and trigger a response and may remain in the body for a long time. The response may involve a) other lymphocytes and b) any of the other white blood cells (leukocytes)

EVIDENTIAL VALIDATION:

The foolproof evidence for the proven efficacy of our herbal medicine HOO- IMM Plus is as elucidated below: -

1) 6ml serum / blood sample is to be drawn from a person previously infected with HIV/AIDS and undergone the complete course of treatment with our medicine HOO-IMM Plus.

2) The sample is to be well handled as per the NABL guidelines and to be sent to a renowned and reliable PCR Molecular diagnostic lab.

3) 3 ml of the sample is to be put for Western Blot Antibody test and the other 3 ml for HIV DNA-PCR Antigen test.

4) The result of the above mentioned tests would report that the blood sample is positive for Western blot Antibody test & Negative for HIV DNA-PCR Antigen test.

5) This Interprets that at present the person is HIV negative though he still carries HIV antibodies (which is not harmful to health), authenticating his earlier infection with HIV virus.

6) The scientific reason behind this reporting is that our body immune system creates cell mediated immunity responses towards HIV antigen to produce antibodies that may persist for a long time, even after the antigen is completely eliminated from the body. The other best example is Mycobacterium Tuberculosis antibodies.

The human body can respond to antigen in many different ways. These fall into two major categories:

· Antibody-mediated immunity. Antibodies, dissolved in blood, lymph, and other body fluids bind the antigen and trigger a response to it. (This form of immunity is also called humoral immunity).

· Cell-mediated immunity (CMI). T cells (lymphocytes) bind to the surface of other cells, display the antigen and trigger a response and may remain in the body for a long time. The response may involve a) other lymphocytes and b) any of the other white blood cells (leukocytes)

7) Thus the above mentioned persons who are free from HIV antigen can, now freely marry and indulge in sexual activities without any risk of infection to their life partner, women can conceive without any chance of transmitting the disease to children (though antibodies are likely to be transmitted between the marital partners or from mother to fetus, it is not harmful to health) and lead a very normal life.

(It seems that many of the laboratories and doctors are still unaware of this latest molecular diagnostic test)